Diploid Mouse Parthenogenones: Effect of Oocyte Aging in Vivo
نویسندگان
چکیده
Mouse oocytes were collected 13.5-29,5hr after hCG injection at 4hr intervals, then activated with 7% ethanol for lmin. The oocytes col!ected 13.5hr after hCG injection did not respond to ethanol activation. High activation rates {90,2-98.3%) were obtained in oocytes activated 17.5-29.5hr after iniection with hCG. Haploid parthenogenones were dominant though the nurnber decreased as the age of oocyte$ advanced (89.5% to 42.0%). The highest number of diploid parthenogenones (20,6%) was obtained in oocytes activated 21.5hr after hCG iniection. The number of immediate cleavage and morphologically abnormal oocytes increased when the oocyte age progressed (O,7% to 34,7% and 1,1% to 23.2%, respective!y). The percentage ot parthenogenones developing to blastocysts decreased with the increase in oocytes age : haploid 51.9% to 1.4% and diploid 100.0% to 83.3%. The present study demonstrates that the developmental potentials of haploid and diploid parthenogenones derived from oocytes at 17.5 and 21.5hr are higher than those derived from oocytes at 25.5 and 29.5hr after hCG injection.
منابع مشابه
Development of haploid and diploid mouse parthenogenones: effect of oocyte aging in vivo.
Mouse oocytes were collected 13.5-29.5 hr after hCG injection at 4 hr intervals, then activated with 7% ethanol for 1 min. The oocytes collected 13.5 hr after hCG injection did not respond to ethanol activation. High activation rates (90.2-98.3%) were obtained in oocytes activated 17.5-29.5 hr after injection with hCG. Haploid parthenogenones were dominant though the number decreased as the age...
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